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Ezrin Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining in human placenta and skeletal muscle tissues using anti-EZR antibody. Corresponding EZR RNA-seq data are presented for ...read more
Genetic Strategies: Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in human placenta tissue.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP1-86863] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.
Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human lymph node shows strong membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Ezrin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Ezrin Recombinant Protein Antigen (NBP1-86863PEP)
Publications
Read Publications using
NBP1-86863 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ezrin Antibody

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Background

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ezrin Antibody (NBP1-86863)(2)

Reviews for Ezrin Antibody (NBP1-86863) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ezrin Antibody (NBP1-86863) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Ezrin Products

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Blogs on Ezrin

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Bioinformatics

Gene Symbol EZR