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EWSR1 Antibody

Images

 
Western Blot: EWSR1 Antibody [NBP2-16389] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EWSR1 antibody diluted at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: EWSR1 Antibody [NBP2-16389] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse duodenum. EWSR1 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded rat fore brain. EWSR1 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse testis. EWSR1 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse prostate. EWSR1 antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EWSR1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EWSR1. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm; Cell membrane
Predicted Species
Porcine (98%), Chimpanzee (100%), Bovine (97%), Rhesus Macaque (100%), Canine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EWSR1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:3000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for EWSR1 Antibody

  • bK984G1.4
  • Ewing sarcoma breakpoint region 1 protein
  • Ewing sarcoma breakpoint region 1
  • Ewings sarcoma EWS-Fli1 (type 1) oncogene
  • EWS oncogene
  • EWS
  • EWSR1
  • EWSRNA-binding protein EWS

Background

This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EWSR1
Uniprot