![Western Blot: FLI1 Antibody [NBP2-16500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FLI1-Antibody-Western-Blot-NBP2-16500-img0014.jpg "Western Blot: FLI1 Antibody [NBP2-16500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody diluted at 1:1000.")
| Reactivity | Hu, Rt, Bv, ChSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Chicken (91%), Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FLI1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets-1_AF7284_Western_Blot_11854.jpg)
![Immunohistochemistry Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets1_AF7284_Immunohistochemistry_22698.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for FLI1 Antibody (NBP2-16500)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: FLI1 Antibody [NBP2-16500] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLI1 protein stained by FLI1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/FLI1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16500-img0003.jpg "Immunocytochemistry/Immunofluorescence: FLI1 Antibody [NBP2-16500] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLI1 protein stained by FLI1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.")
![Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/FLI1-Antibody-Immunohistochemistry-Paraffin-NBP2-16500-img0002.jpg "Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.")
![Western Blot: FLI1 Antibody [NBP2-16500] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGE FLI1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/FLI1-Antibody-Western-Blot-NBP2-16500-img0010.jpg "Western Blot: FLI1 Antibody [NBP2-16500] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGE FLI1 antibody dilution: 1:1000")
![Western Blot: FLI1 Antibody [NBP2-16500] - A. 30 ug BCL-1 whole cell lysate/extract 10 % SDS-PAGEFLI1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/FLI1-Antibody-Western-Blot-NBP2-16500-img0012.jpg "Western Blot: FLI1 Antibody [NBP2-16500] - A. 30 ug BCL-1 whole cell lysate/extract 10 % SDS-PAGEFLI1 antibody dilution: 1:1000")
![Immunoprecipitation: FLI1 Antibody [NBP2-16500] - IP samples: Raji whole cell extract A. 40 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 ug FLI1 antibody (NBP2-16500) 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody (NBP2-16500) diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/FLI1-Antibody-Immunoprecipitation-NBP2-16500-img0006.jpg "Immunoprecipitation: FLI1 Antibody [NBP2-16500] - IP samples: Raji whole cell extract A. 40 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 ug FLI1 antibody (NBP2-16500) 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody (NBP2-16500) diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
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![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")