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ESAM Antibody [Unconjugated]

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bEnd.3 cells were stained with Goat Anti-Mouse ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2827, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
bEnd.3 cells were stained with Goat Anti-Mouse ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2827, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed ...read more
ESAM was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # af2827) at 5 µg/ml for 1 hour at room temperature ...read more
ESAM was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # af2827) at 5 µg/ml for 1 hour at room temperature ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2827
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2827-SP
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ESAM Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ESAM
Gln30-Ser248 (predicted)
Accession # Q925F2
Specificity
Detects mouse ESAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human ESAM is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ESAM
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2827 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ESAM Antibody [Unconjugated]

  • 2310008D05Rik
  • endothelial cell adhesion molecule
  • endothelial cell-selective adhesion molecule
  • ESAM
  • HUEL (C4orf1)-interacting protein
  • LP4791 protein
  • W117m

Background

ESAM is a 55 kDa type I transmembrane glycoprotein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members are CXADR, CLMP, JAM-A-C, CD2, A33, and BT-IgSF. The extracellular region of ESAM contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion. Mouse ESAM extracellular domain shares 69% amino acid sequence identity with the corresponding region of human ESAM. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes and can be found associated with cell-to-cell junctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ESAM Antibody (AF2827)(3)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 2 applications: CITE-seq, IHC.


Filter By Application
CITE-seq
(1)
IHC
(1)
All Applications
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Mouse
(1)
Transgenic Mouse
(1)
All Species

Reviews for ESAM Antibody (AF2827) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ESAM Antibody (AF2827) (0)

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Secondary Antibodies

 

Isotype Controls

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Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol ESAM
Uniprot