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ESAM Antibody (408519) [Unconjugated]

Images

 
HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), ...read more
ESAM was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
408519
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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ESAM Antibody (408519) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ESAM
Gln30-Ala247
Accession # Q96AP7
Specificity
Detects human ESAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ESAM is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ESAM
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using MAB4204.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ESAM Antibody (408519) [Unconjugated]

  • 2310008D05Rik
  • endothelial cell adhesion molecule
  • endothelial cell-selective adhesion molecule
  • ESAM
  • HUEL (C4orf1)-interacting protein
  • LP4791 protein
  • W117m

Background

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69%, and 67% aa identity with monkey, canine, mouse, and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets, and megakaryocytes and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

  1. Hirata, K-I. et al. (2001) J. Biol. Chem. 276:16223.
  2. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.
  3. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.
  4. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ESAM Antibody (MAB4204)(2)

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Bioinformatics

Gene Symbol ESAM
Uniprot