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ERCC8 Antibody

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Western Blot: ERCC8 Antibody [NBP1-32166] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC8 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ERCC8 Antibody [NBP1-32166] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC8 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: ERCC8 Antibody [NBP1-32166] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Western Blot: ERCC8 Antibody [NBP1-32166] - A. Neuro2A whole cell lysate/extract. B. GL261 whole cell lysate/extract. C. C8D30 whole cell lysate/extract. D. NIH-3T3 whole cell lysate/extract. E. Raw264.7 whole cell ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Func, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ERCC8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ERCC8. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERCC8
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
Functional Assay- Assay dependent
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for ERCC8 Antibody

  • CKN1DNA excision repair protein ERCC-8
  • Cockayne syndrome 1 (classical)
  • Cockayne syndrome WD repeat protein CSA
  • CSACockayne syndrome WD-repeat protein CSA
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 8

Background

This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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NER Antibodies in Cancer Research
We at Novus Biologicals have over 230 products in our antibody catalog devoted to nucleotide excision repair. NER is a multi-stage sequential process involving over 30 proteins, all of which have been widely studied. Being the primary method to repair...  Read full blog post.
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Bioinformatics

Gene Symbol ERCC8