![Western Blot: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPLIN.](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Western-Blot-NBP2-71932-img0001.jpg "Western Blot: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPLIN.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI1A3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-275 of human LIMA1(NP_057441) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LIMA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 85 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LIMA1 |
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0009.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0002.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0003.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0004.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0005.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0006.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0007.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0008.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71932-img0010.jpg "Immunohistochemistry: EPLIN Antibody (OTI1A3) - Azide and BSA Free [NBP2-71932] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Immunohistochemistry Leptin R Antibody [Unconjugated]](https://images.novusbio.com/images2/Leptin_R_AF497_Immunohistochemistry_6850.jpg)
![Western Blot Leptin R Antibody [Unconjugated]](https://images.novusbio.com/images/af497_mouse-leptin-r-affinity-purified-polyclonal-ab-41202410481130.jpg)
![Simple Western Vinculin Antibody (728526) [Unconjugated]](https://images.novusbio.com/images2/Vinculin_MAB6896_Simple_Western_16408.jpg)
![Western Blot Vinculin Antibody (728526) [Unconjugated]](https://images.novusbio.com/images2/Vinculin_MAB6896_Western_Blot_11566.jpg)
![Immunohistochemistry Vinculin Antibody (728526) [Unconjugated]](https://images.novusbio.com/images2/Vinculin_MAB6896_Immunohistochemistry_11621.jpg)
![Western Blot HAX-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/HAX-1_AF5458_Western_Blot_5727.jpg)
![Immunocytochemistry HAX-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/HAX1_AF5458_Immunocytochemistry__Immunofluorescence_18999.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")