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Ephrin-A5 Antibody

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Immunocytochemistry/Immunofluorescence: Ephrin-A5 Antibody [NBP2-58251] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Ephrin-A5 Antibody [NBP2-58251] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Ephrin-A5 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: FYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVHDRVFDVNDKVENSLEPADDTVHESAEPSRGENAAQ
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFNA5
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot Reported in scientific literature (PMID 28169277)
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Ephrin-A5 Recombinant Protein Antigen (NBP2-58251PEP)
Reviewed Applications
Read 1 Review rated 1
using
NBP2-58251 in the following applications:
Publications
Read Publication using
NBP2-58251 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28169277).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Ephrin-A5 Antibody

  • AF1
  • AL-1
  • EFL-5
  • EFNA5
  • EPH-related receptor tyrosine kinase ligand 7
  • EphrinA5
  • Ephrin-A5
  • EPLG7
  • GLC1M
  • LERK-7
  • LERK7EFL5
  • RAGS

Background

Ephrin A5, a member of the ephrin family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Ephrin-A5 Antibody
NBP2-58251
Ephrin-A5 Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF

Publications for Ephrin-A5 Antibody (NBP2-58251)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Review for Ephrin-A5 Antibody (NBP2-58251) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-58251:
Filter by Applications
IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  1
reviewed by:
Verified Customer
 IHC-Fr Mouse 07/28/2021
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse lens tissue
SpeciesMouse
LotR72040

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ephrin-A5 Antibody (NBP2-58251) (0)

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Secondary Antibodies

 

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Immunohistochemistry-Frozen Ephrin-A5 NBP2-58251
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 Verified Customer
07/28/2021
Application: IHC-Fr
Species: Mouse
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Bioinformatics

Gene Symbol EFNA5