![Western Blot: EPB41L5 Antibody [NBP2-38354] - Analysis in control (vector only transfected HEK293T lysate) and EPB41L5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/EPB41L5-Antibody-Western-Blot-NBP2-38354-img0006.jpg "Western Blot: EPB41L5 Antibody [NBP2-38354] - Analysis in control (vector only transfected HEK293T lysate) and EPB41L5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: DLLEATIGDVIGASDTMETSQALNDVNVATRLPGLGEPEVEYETLKDTSEKLKQLEMENSPLLSPRSNIDVNINSQEEVVKLTEKC |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EPB41L5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP2-38354 | Applications | Species |
|---|---|---|
| Troyanovsky RB, Indra I, Kato R Et al. Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity The Journal of biological chemistry 2021-10-08 [PMID: 34634305] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: EPB41L5 Antibody [NBP2-38354] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EPB41L5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38354-img0004.jpg "Immunocytochemistry/Immunofluorescence: EPB41L5 Antibody [NBP2-38354] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/EPB41L5-Antibody-Immunohistochemistry-Paraffin-NBP2-38354-img0010.jpg "Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/EPB41L5-Antibody-Immunohistochemistry-Paraffin-NBP2-38354-img0007.jpg "Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.")
![Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/EPB41L5-Antibody-Immunohistochemistry-Paraffin-NBP2-38354-img0008.jpg "Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize/EPB41L5-Antibody-Immunohistochemistry-Paraffin-NBP2-38354-img0009.jpg "Immunohistochemistry-Paraffin: EPB41L5 Antibody [NBP2-38354] - Staining of human skeletal muscle shows very weak positivity in myocytes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")