![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Western-Blot-NBP2-67641-img0007.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | SC06-28 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human Enolase 2/Neuron-specific Enolase aa 30-70. (SwissProt: P09104 Human; SwissProt: P17183 Mouse; SwissProt: P07323 Rat) |
| Localization | Cytoplasm, Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ENO2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Enolase 2/Neuron-specific Enolase Antibody (NBP2-67641)Find related products by research area.
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| Gene Symbol | ENO2 |
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunocytochemistry-Immunofluorescence-NBP2-67641-img0002.jpg "Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunohistochemistry-Paraffin-NBP2-67641-img0005.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin.")
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was use](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Western-Blot-NBP2-67641-img0008.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was use")
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunocytochemistry-Immunofluorescence-NBP2-67641-img0001.jpg "Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Analysis of paraffin-embedded mouse brain tissue using anti-NSE antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SC06-28-Immunohistochemistry-Paraffin-NBP2-67641-img0004.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Analysis of paraffin-embedded mouse brain tissue using anti-NSE antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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