![Western Blot: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Azide-and-BSA-Free-Western-Blot-NBP2-80719-img0002.jpg "Western Blot: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 3A9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human CD105 expressed in E. coli. [Uniprot: P17813] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ENG |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 71 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Endoglin/CD105 Antibody (NBP2-80719)Find related products by research area.
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| Gene Symbol | ENG |
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - HMSC cells stained with anti-CD105 antibody. Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80719-img0004.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - HMSC cells stained with anti-CD105 antibody. Image from verified customer review. Image from the standard format of this antibody.")
![Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80719-img0005.jpg "Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining. Image from the standard format of this antibody.")
![Western Blot: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Azide-and-BSA-Free-Western-Blot-NBP2-80719-img0001.jpg "Western Blot: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80719-img0003.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). Image from the standard format of this antibody.")
![ELISA: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Azide-and-BSA-Free-ELISA-NBP2-80719-img0006.jpg "ELISA: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")