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EN1/Engrailed 1 Antibody

Images

 
Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332] - Mouse spinal cord. EN1/Engrailed 1 stained by EN1/Engrailed 1 antibody (NBP3-13332) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 ...read more
Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332] - Mouse brain. EN1/Engrailed 1 stained by EN1/Engrailed 1 antibody (NBP3-13332) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Frozen: EN1/Engrailed 1 Antibody [NBP3-13332] - EN1/Engrailed 1 antibody detects EN1/Engrailed 1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EN1/Engrailed 1 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse EN1/Engrailed 1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EN1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for EN1/Engrailed 1 Antibody

  • engrailed homeobox 1
  • engrailed homolog 1
  • Homeobox protein en-1
  • homeobox protein engrailed-1
  • hu-En-1

Background

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Array NBP3-13332

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Bioinformatics

Gene Symbol EN1