![Immunoprecipitation: ELYS Antibody [NB600-238] - Nuclear Extract (10 and 20 ug for WB; 1 mg for IP/WB) from HeLa cells. Antibodies: A) Affinity purified rabbit anti-ELYS antibody BL476 used at 0.5 ug/ml for WB and 4 ug/mg lysate for IP. B) Affinity purified rabbit anti-ELYS antibodies BL476 and BL475 were each used at 4 ug/mg extract for IP. Immunoprecipitated ELYS was detected by WB using BL475 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 3 to 5 minutes.](http://images.novusbio.com/fullsize/ELYS-Antibody-Immunoprecipitation-NB600-238-img0003.jpg "Immunoprecipitation: ELYS Antibody [NB600-238] - Nuclear Extract (10 and 20 ug for WB; 1 mg for IP/WB) from HeLa cells. Antibodies: A) Affinity purified rabbit anti-ELYS antibody BL476 used at 0.5 ug/ml for WB and 4 ug/mg lysate for IP. B) Affinity purified rabbit anti-ELYS antibodies BL476 and BL475 were each used at 4 ug/mg extract for IP. Immunoprecipitated ELYS was detected by WB using BL475 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 3 to 5 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A syntetic peptide between aa2220 and C-term |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AHCTF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Simple Western reported in scientific literature (PMID:35017527) By Western Blot, this antibody recognizes a band at ~252.5 kDa, representing ELYS protein. May be used for immunoprecipitation, as well. Western blot of immunoprecipitates was performed using an immunoprecipitation/Western blot reagent and 4-8% SDS-PAGE. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publications using NB600-238 | Applications | Species |
|---|---|---|
| Lewandowska Ronnegren A Crosstalk between environmental signals and 3d genome organization in the regulation of gene expression Thesis 2021-01-01 (IP) | IP | |
| Chachoua I, Tzelepis I, Dai H et al. Canonical WNT signaling-dependent gating of MYC requires a noncanonical CTCF function at a distal binding site Nature communications 2022-01-11 [PMID: 35017527] (Simple Western, Human) | Simple Western | Human |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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