Novus Biologicals products are now on bio-techne.com

ELOVL1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ELOVL1 Antibody [NBP2-37956] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining in human skin and skeletal muscle tissues . Corresponding ELOVL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human skin shows strong granular cytoplasmic positivity in sebaceous gland and keratinocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

ELOVL1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FWYHSYTKGKRLPRALQQNGAPGIAKVK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELOVL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ELOVL1 Protein (NBP2-37956PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ELOVL1 Antibody

  • EC 2.3.1.n8
  • elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1
  • elongation of very long chain fatty acids protein 1
  • SSC1
  • Ssc1,3-keto acyl-CoA synthase ELOVL1

Background

Elongation of very long chain fatty acid-like (ELOVL) proteins 1-6 are members of the ELO family of proteins, which play an important role in tissue-specific biosynthesis of very long chain fatty acids and sphingolipids. The ELOVL proteins act as catalysts in fatty acid elongation reduction and localize to the endoplasmic reticulum (ER). Elongation of very long chain fatty acids protein 1 (ELOVL1), also referred to as Ssc1, is the human homolog of the yeast ELO3 protein. It is expressed in a variety of tissues and at especially high levels in stomach, skin, intestine, kidney and lung. ELOVL1 participates in the elongation of very long chain saturated and monounsaturated fatty acids of up to 26 carbons and may be required for the development of a barrier in epithelial cells and skin. ELOVL1 is also important for the formation of Myelin in the central nervous system. Impaired ELOVL1 activity may be associated with disorders of sphingolipid metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-81696
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00054898-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-76566
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-46476
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, KD, KO, WB
NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00008458-M06
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP1-86941
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-32629
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-52823
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-91642
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00010049-M01
Species: Hu
Applications: ELISA, ICC/IF (-), IHC, WB
NBP2-02996
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
G-125-C
Species: Hu
Applications:
NBP1-77397
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
NB400-114
Species: Ch, Fe, Ha, Hu, Mu, Pl, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
NBP2-92633
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NB300-543
Species: Am, Bv, Ca, Ma, Fi, Hu, Ma-Mn, Mu, Pm, Rb, Rt, Sh
Applications: B/N, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP3-17923
Species: Hu
Applications: IHC, IHC-P
NBP2-84247
Species: Hu
Applications: WB
NBP2-37956
Species: Hu
Applications: ICC/IF, IHC

Publications for ELOVL1 Antibody (NBP2-37956) (0)

There are no publications for ELOVL1 Antibody (NBP2-37956).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ELOVL1 Antibody (NBP2-37956) (0)

There are no reviews for ELOVL1 Antibody (NBP2-37956). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for ELOVL1 Antibody (NBP2-37956) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ELOVL1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ELOVL1
Uniprot