![Western Blot: eIF4ENIF1 Antibody [NB100-1368] - Analysis of eIF4ENIF1 (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/eIF4ENIF1-Antibody-Western-Blot-NB100-1368-img0003.jpg "Western Blot: eIF4ENIF1 Antibody [NB100-1368] - Analysis of eIF4ENIF1 (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-AKVISVDELEYRQ corresponding to C-Terminus according to NP_062817.2, NP_001157974.1. |
| Localization | Cytoplasmic |
| Specificity | This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1). |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | EIF4ENIF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 140-150 kDa band observed in 293 lysates (predicted MW of approx. 108 kDa band according to NP_062817.1) and is in agreement with scientific literature (Dostie et al). IHC-P: Human pancreas shows strong staining of cytoplasm in acinar cells-. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-1368 | Applications | Species |
|---|---|---|
| Dostie J, Ferraiuolo M, Pause A et al. A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E. EMBO J 2000-06-15 [PMID: 10856257] |
Secondary Antibodies |
Isotype Controls |
Research Areas for eIF4ENIF1 Antibody (NB100-1368)Find related products by research area.
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| Gene Symbol | EIF4ENIF1 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry: eIF4ENIF1 Antibody [NB100-1368] - IHC analysis of eIF4ENIF1 in paraffin embedded Human Pancreas using this antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/eIF4ENIF1-Antibody-Immunohistochemistry-NB100-1368-img0001.jpg "Immunohistochemistry: eIF4ENIF1 Antibody [NB100-1368] - IHC analysis of eIF4ENIF1 in paraffin embedded Human Pancreas using this antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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