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eIF3K Overexpression Lysate

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Western Blot: eIF3K Overexpression Lysate (Adult Normal) [NBP2-07433] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF3K.
Western Blot: eIF3K Overexpression Lysate (Adult Normal) [NBP2-07433] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF3K.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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eIF3K Overexpression Lysate Summary

Description

eIF3K Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC203529

Accession#: NM_013234

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
EIF3K

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for eIF3K Overexpression Lysate

  • ARG134
  • eIF3k
  • EIF3-p28
  • EIF3S12eIF-3 p28
  • Eukaryotic translation initiation factor 3 subunit 12
  • eukaryotic translation initiation factor 3 subunit K
  • eukaryotic translation initiation factor 3, subunit 12
  • eukaryotic translation initiation factor 3, subunit K
  • HSPC029
  • M9
  • MSTP001
  • muscle specific
  • Muscle-specific gene M9 protein
  • PLAC24
  • PLAC-24
  • PLAC-24eIF-3 p25
  • PRO1474
  • PTD001

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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eIF3K
Overexpression Lysate
NBP2-07433
eIF3K Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol EIF3K