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![Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NB100-93304-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-310202416284928.jpg)
![Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-31020241628498.jpg)
![Western Blot: eIF3K Antibody [NBP1-91865] - Analysis using Anti-EIF3K antibody NBP1-91865 (A) shows similar pattern to independent antibody NBP2-56764 (B).](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NBP1-91865-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eIF3K Antibody [NBP1-91865] - Analysis using Anti-EIF3K antibody NBP1-91865 (A) shows similar pattern to independent antibody NBP2-56764 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: eIF3K Antibody [NBP1-91865] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Immunohistochemistry-Paraffin-NBP1-91865-img0008.jpg)
![Western Blot: eIF3K Antibody [NBP2-56764] - Analysis using Anti-EIF3K antibody NBP2-56764 (A) shows similar pattern to independent antibody NBP1-91865 (B).](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NBP2-56764-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eIF3K Antibody [NBP2-56764] - Analysis using Anti-EIF3K antibody NBP2-56764 (A) shows similar pattern to independent antibody NBP1-91865 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: eIF3K Antibody [NBP2-56764] - Staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56764-img0001.jpg)
![Western Blot: eIF3K Antibody [NBP1-31285] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NBP1-31285-img0003.jpg "Western Blot: eIF3K Antibody [NBP1-31285] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: eIF3K Antibody [NBP1-31285] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3K protein stained by EIF3K antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31285-img0002.jpg)
![Western Blot: eIF3K Antibody [NBP1-98396] - Mouse Liver lysate, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NBP1-98396-img0002.jpg "Western Blot: eIF3K Antibody [NBP1-98396] - Mouse Liver lysate, concentration 1 ug/ml.")
![Western Blot: eIF3K Overexpression Lysate (Adult Normal) [NBP2-07433] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF3K.](http://images.novusbio.com/fullsize/eIF3K-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07433-img0001.jpg "Western Blot: eIF3K Overexpression Lysate (Adult Normal) [NBP2-07433] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF3K.")
![Immunocytochemistry/Immunofluorescence: eIF3K Antibody (01) [NBP3-06567] - Staining of Human EIF3K in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EIF3K monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/eIF3K-Antibody-01-Immunocytochemistry-Immunofluorescence-NBP3-06567-img0001.jpg "Immunocytochemistry/Immunofluorescence: eIF3K Antibody (01) [NBP3-06567] - Staining of Human EIF3K in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EIF3K monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.")
![SDS-Page: Recombinant Human eIF3K Protein [NBP1-51042] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-eIF3K-Protein-SDS-Page-NBP1-51042-img0001.jpg "SDS-Page: Recombinant Human eIF3K Protein [NBP1-51042] - 15 % SDS-PAGE (3ug)")