![Western Blot: EEN Antibody (2F5) [NBP2-02834] Analysis of extracts (35ug) from 9 different cell lines by using anti-EEN monoclonal antibody.](http://images.novusbio.com/fullsize/EEN-Antibody-2F5-Western-Blot-NBP2-02834-img0006.jpg "Western Blot: EEN Antibody (2F5) [NBP2-02834] Analysis of extracts (35ug) from 9 different cell lines by using anti-EEN monoclonal antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.64 mg/ml |
| Immunogen | Full length human recombinant protein of human SH3GL1 (NP_003016) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SH3GL1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 41.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.64 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: EEN Antibody (2F5) [NBP2-02834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EEN.](http://images.novusbio.com/fullsize/EEN-Antibody-2F5-Immunocytochemistry-Immunofluorescence-NBP2-02834-img0005.jpg "Immunocytochemistry/Immunofluorescence: EEN Antibody (2F5) [NBP2-02834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EEN.")
![Immunohistochemistry-Paraffin: EEN Antibody (2F5) [NBP2-02834] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EEN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EEN-Antibody-2F5-Immunohistochemistry-Paraffin-NBP2-02834-img0004.jpg "Immunohistochemistry-Paraffin: EEN Antibody (2F5) [NBP2-02834] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EEN mouse monoclonal antibody.")
![Flow Cytometry: EEN Antibody (2F5) [NBP2-02834] - Analysis of Jurkat cells, using anti-EEN antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/EEN-Antibody-2F5-Flow-Cytometry-NBP2-02834-img0002.jpg "Flow Cytometry: EEN Antibody (2F5) [NBP2-02834] - Analysis of Jurkat cells, using anti-EEN antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: EEN Antibody (2F5) [NBP2-02834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EEN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EEN.](http://images.novusbio.com/fullsize/EEN-Antibody-2F5-Western-Blot-NBP2-02834-img0003.jpg "Western Blot: EEN Antibody (2F5) [NBP2-02834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EEN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EEN.")
![Flow Cytometry: EEN Antibody (2F5) [NBP2-02834] - Analysis of Hela cells, using anti-EEN antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/EEN-Antibody-2F5-Flow-Cytometry-NBP2-02834-img0001.jpg "Flow Cytometry: EEN Antibody (2F5) [NBP2-02834] - Analysis of Hela cells, using anti-EEN antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")