![Western Blot: EEN Antibody (4B4C2) [NBP2-52561] - Western blot analysis using EEN mouse mAb against HepG2 (1), A549 (2), HT-29 (3), and PC-12 (4) cell lysate.](http://images.novusbio.com/fullsize/EEN-Antibody-4B4C2-Western-Blot-NBP2-52561-img0004.jpg "Western Blot: EEN Antibody (4B4C2) [NBP2-52561] - Western blot analysis using EEN mouse mAb against HepG2 (1), A549 (2), HT-29 (3), and PC-12 (4) cell lysate.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, CyTOF-ready |
| Clone | 4B4C2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human EEN (AA: 12-119) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SH3GL1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 41.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SH3GL1 |
![Western Blot: EEN Antibody (4B4C2) [NBP2-52561] - Analysis using SH3GL1 mAb against human SH3GL1 (AA: 12-119) recombinant protein. (Expected MW is 37.6 kDa)](http://images.novusbio.com/fullsize/EEN-Antibody-4B4C2-Western-Blot-NBP2-52561-img0001.jpg "Western Blot: EEN Antibody (4B4C2) [NBP2-52561] - Analysis using SH3GL1 mAb against human SH3GL1 (AA: 12-119) recombinant protein. (Expected MW is 37.6 kDa)")
![Western Blot: EEN Antibody (4B4C2) [NBP2-52561] - Analysis using SH3GL1 mAb against HEK293 (1) and SH3GL1 (AA: 12-119)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/EEN-Antibody-4B4C2-Western-Blot-NBP2-52561-img0002.jpg "Western Blot: EEN Antibody (4B4C2) [NBP2-52561] - Analysis using SH3GL1 mAb against HEK293 (1) and SH3GL1 (AA: 12-119)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: EEN Antibody (4B4C2) [NBP2-52561] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/EEN-Antibody-4B4C2-ELISA-NBP2-52561-img0003.jpg "ELISA: EEN Antibody (4B4C2) [NBP2-52561] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).")
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")