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EEF2K Antibody

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Western Blot: EEF2K Antibody [NBP3-23302] - Western blot image of EEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of EEF2K from HeLa cell lysate using anti-EEF2K (C-terminus) antibody (lanes 4 & 6). Negative control immunopreciptations with no antibody are shown in lane 5 and 7. The blots were probed with anit-EEF2K (C-terminus) (lanes 1-5) or with anti-EEF2K (N-terminal) antibody (lanes 6 & 7).
Western Blot: EEF2K Antibody [NBP3-23302] - Western blot image of EEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of EEF2K from HeLa cell lysate using anti-EEF2K ...read more
Immunocytochemistry/ Immunofluorescence: EEF2K Antibody [NBP3-23302] - Immunocytochemical labeling of EEF2K in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rabbit polyclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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EEF2K Antibody Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
eEF2K (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human eEF2K. This sequence is well conserved in rat, mouse, chicken, and fish eEF2K, and has low homology to other proteins. [UniProt# O00418]
Specificity
The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human A431, HeLa, and Jurkat cells. The antibody also detected eEF2K in immunoprecipitations in HeLa cells and immunofluorescent labeling in A431 cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEF2K
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunoprecipitation 1:100
  • Western Blot 1:1000
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Antigen Affinity-purified

Alternate Names for EEF2K Antibody

  • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
  • calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
  • EC 2.7.11
  • EC 2.7.11.20
  • eEF-2 kinase
  • eEF-2Kcalmodulin-dependent protein kinase III
  • elongation factor-2 kinase
  • eukaroytic elongation factor 2 kinase
  • eukaryotic elongation factor 2 kinase
  • eukaryotic elongation factor-2 kinase
  • HSU93850
  • MGC45041

Background

EEF2K encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EEF2K