![Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Western-Blot-NBP2-53354-img0006.jpg "Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA |
| Clone | CDH1/1525 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54551) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant human E-Cadherin protein (Uniprot: P12830) |
| Localization | Cell Surface |
| Marker | Intercellular Junction Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDH1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunocytochemistry-Immunofluorescence-NBP2-53354-img0007.jpg "Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.")
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