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Dysferlin Antibody (SR2944)

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Western Blot: Dysferlin Antibody (SR2944) [NBP3-27723] - Western blot analysis of Dysferlin expression in Mouse skeletal muscle tissue lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR2944
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Dysferlin Antibody (SR2944) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human Dysferlin (Uniprot #: O75923)
Specificity
Detects endogenous levels of Dysferlin
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DYSF
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 150mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Dysferlin Antibody (SR2944)

  • dysferlin
  • dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)
  • dystrophy-associated fer-1-like 1
  • FER1L1Dystrophy-associated fer-1-like protein
  • Fer-1-like protein 1
  • FLJ00175
  • FLJ90168
  • LGMD2B

Background

Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dysferlin Antibody (NBP3-27723) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DYSF