![Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Otoferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-46574-img0005.jpg "Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | A synthetic peptide from amino acid region 350-400 of human Otoferlin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | OTOF |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from whole antisera |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Unpurified |
| Reconstitution Instructions | Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
![Cochlin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Cochlin [Unconjugated]")
| Publication using NBP1-46574 | Applications | Species |
|---|---|---|
| van der Valk WH, van Beelen ESA, Steinhart MR et al. A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs Cell reports 2023-06-06 [PMID: 37289589] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - Rat cerebellum. Dilution 1: 1000.](http://images.novusbio.com/fullsize/Otoferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-46574-img0001.jpg "Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - Rat cerebellum. Dilution 1: 1000.")
![Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - Rat spinal cord, dilution 1: 1000.](http://images.novusbio.com/fullsize/Otoferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-46574-img0002.jpg "Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - Rat spinal cord, dilution 1: 1000.")
![Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Otoferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-46574-img0003.jpg "Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Otoferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-46574-img0004.jpg "Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Otoferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-46574-img0006.jpg "Immunohistochemistry-Paraffin: Otoferlin Antibody [NBP1-46574] - IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
![Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]](https://images.novusbio.com/images2/Synaptotagmin-1_MAB4364_Immunocytochemistry_11323.jpg)
![Immunohistochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]](https://images.novusbio.com/images2/Synaptotagmin-1_MAB4364_Immunohistochemistry_7025.jpg)
![Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]](https://images.novusbio.com/images2/Synaptotagmin1_MAB4364_Immunocytochemistry_13008.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")