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DPPA2 Antibody

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DPPA2 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human DPPA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7086) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

DPPA2 Antibody Summary

Immunogen
E. coli-derived recombinant human DPPA2
Ser2-Thr174
Accession # Q7Z7J5
Specificity
Detects human DPPA2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
DPPA2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPA2 Antibody

  • CT100
  • developmental pluripotency associated 2
  • developmental pluripotency-associated protein 2
  • DPPA2
  • ECSA
  • PESCRG1
  • PESCRG1cancer/testis antigen 100
  • Pluripotent embryonic stem cell-related gene 1 protein

Background

DPPA2 (Developmental pluripotency associated 2; also ESCA, CT100 and PESCRG1) is a 38-39 kDa member of both the DPPA and cancer-testis family of proteins. It is found in pluripotent stem cells in a pattern that parallels that of Oct4, and likely contributes to formation of the ICM, plus the maintainence of cells in an undifferentiated state. DPPA2 is also found in spermatocytes and select tumor types. Human DPPA2 is 298 amino acids (aa) in length. It contains one DNA-binding SAP domain that is involved in chromosomal organization (aa 92-126). One potential isoform variant has been reported that shows a deletion of aa 61-71. Over aa 2‑174, human DPPA2 shares only 36% aa identity with mouse DPPA2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DPPA2
Uniprot