![Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/DNA-Polymerase-epsilon-catalytic-subunit-A-Antibody-Western-Blot-NBP3-13067-img0003.jpg "Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DNA Polymerase epsilon catalytic subunit A. The exact sequence is proprietary. |
| Predicted Species | Mouse (90%), Rat (90%), Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | POLE |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for DNA Polymerase epsilon catalytic subunit A Antibody (NBP3-13067)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | POLE |
![Immunocytochemistry/Immunofluorescence: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA Polymerase epsilon catalytic subunit A protein stained by DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:2500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/DNA-Polymerase-epsilon-catalytic-subunit-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13067-img0002.jpg "Immunocytochemistry/Immunofluorescence: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA Polymerase epsilon catalytic subunit A protein stained by DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:2500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/DNA-Polymerase-epsilon-catalytic-subunit-A-Antibody-Immunohistochemistry-Paraffin-NBP3-13067-img0001.jpg "Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry MMR/CD206/Mannose Receptor Antibody [Unconjugated]](https://images.novusbio.com/images2/MMR_AF2535_Immunohistochemistry_15450.jpg)
![Western Blot MMR/CD206/Mannose Receptor Antibody [Unconjugated]](https://images.novusbio.com/images2/MMR_AF2535_Western_Blot_12738.jpg)
![Immunohistochemistry MMR/CD206/Mannose Receptor Antibody [Unconjugated]](https://images.novusbio.com/images2/MMR_AF2535_Immunohistochemistry_20447.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")