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POLE4 Antibody

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Immunohistochemistry: POLE4 Antibody [NBP2-49672] - Staining of human kidney shows strong nuclear positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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POLE4 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLE4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
POLE4 Recombinant Protein Antigen (NBP2-49672PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for POLE4 Antibody

  • DNA polymerase epsilon p12 subunit
  • DNA polymerase epsilon subunit 4
  • DNA polymerase epsilon subunit p12
  • DNA polymerase II subunit 4
  • EC 2.7.7.7
  • p12
  • polymerase (DNA-directed), epsilon 4 (p12 subunit)
  • YHHQ1

Background

POLE4, also known as DNA polymerase epsilon subunit 4, is 117 amino acids long and approximately 12kDa. POLE4 localizes to the nucleus. POLE, POLE2, POLE3 and POLE4 are elements of the epsilon DNA polymerase complex (Pol epsilon), and as such, POLE4 plays a role in the process of nuclear DNA replication. POLE4 interacts with POLE, POLE2, and POLE3, as well as UBE3A and HSPA13. POLE4 is implicated in a variety of pathways including the DNA replication pathway, Nucleotide excision repair, Purine metabolism, and cell cycle control of chromosomal replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLE4