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DNA-PKcs Antibody

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Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody [NBP2-33995] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody [NBP2-33995] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DNA-PKcs Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKDC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DNA-PKcs Protein (NBP2-33995PEP)
Publications
Read Publication using
NBP2-33995 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNA-PKcs Antibody

  • DNA-dependent protein kinase catalytic subunit-like
  • DNA-dependent protein kinase
  • DNA-PK catalytic subunit
  • DNAPK
  • DNAPKcs
  • DNA-PKcs
  • DNPK1
  • EC 2.7.11.1
  • HYRC
  • HYRC1
  • p350
  • p460
  • PRKDC
  • XRCC7

Background

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DNA-PKcs Antibody
NBP2-33995
DNA-PKcs Antibody
Species: Hu
Applications: ICC/IF, IHC

Publications for DNA-PKcs Antibody (NBP2-33995)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Immunohistochemistry-Paraffin.


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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKDC
Uniprot