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Dkk-3 Antibody [Unconjugated]

 (5 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF948
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF948-SP
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Dkk-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dkk-3
Met1-Ile349
Accession # Q9QUN9
Specificity
Detects mouse Dkk-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Dkk‑3 is observed and less than 1% cross-reactivity with rhDkk-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKK3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF948 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-3 Antibody [Unconjugated]

  • dickkopf (Xenopus laevis) homolog 3
  • dickkopf 3
  • dickkopf homolog 3 (Xenopus laevis)
  • Dickkopf-3
  • dickkopf-related protein 3
  • Dkk3
  • Dkk-3
  • hDkk-3
  • regulated in glioma
  • REIC
  • REICdkk-3
  • RIG
  • RIG-like 5-6
  • RIG-like 7-1

Background

Dkk-3 (Dickkopf-related protein 3; also REIC) is a 65-70 kDa secreted glycoprotein member of the Dickkopf family of molecules.  It is expressed by multiple cell types, including neurons, endothelial cells, keratinocytes and zona glomerulosa cells of the adrenal.  Dkk-3 is reported to be a positive regulator of Wnt signaling.  It is suggested to bind exclusively to Wnt-inhibiting Kremen proteins, thereby blocking their expression on the cell surface.  This makes LRP available for Wnt binding and signaling.  Dkk-3 is also reported to induce the development of DC from monocytes.  Mature mouse Dkk-3 is 328 amino acids (aa) in length (aa 22-349).  It contains one coiled-coil region (aa 39-84), two consecutive Dkk-type Cys-rich regions (aa 147-195 and 208-284), and one phosphoserine site at Ser298.  Over aa 22-349, mouse shares 83% and 92% aa identity with human and rat Dkk-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Dkk-3 Antibody
[Unconjugated]
AF948
Dkk-3 Antibody [Unconjugated]
Species: Mu
Applications: WB

Publications for Dkk-3 Antibody (AF948)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(2)
Western Blot
(3)
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Mouse
(5)
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Showing Publications 1 - 5 of 5.
Publications using AF948 Applications Species
Martin Flores, N;Podpolny, M;McLeod, F;Workman, I;Crawford, K;Ivanov, D;Leonenko, G;Escott-Price, V;Salinas, PC; Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer's disease, restores synapse integrity and memory in a disease mouse model eLife 2024-01-29 [PMID: 38285009] (IHC, Mouse) IHC Mouse
E Forte, DA Skelly, M Chen, S Daigle, KA Morelli, O Hon, VM Philip, MW Costa, NA Rosenthal, MB Furtado Dynamic Interstitial Cell Response during Myocardial Infarction Predicts Resilience to Rupture in Genetically Diverse Mice Cell Rep, 2020-03-03;30(9):3149-3163.e6. 2020-03-03 [PMID: 32130914] (IHC, Mouse) IHC Mouse
J Yin, L Yang, Y Xie, Y Liu, S Li, W Yang, B Xu, H Ji, L Ding, K Wang, G Li, L Chen, P Hu Dkk3 dependent transcriptional regulation controls age related skeletal muscle atrophy Nat Commun, 2018-05-01;9(1):1752. 2018-05-01 [PMID: 29717119] (Western Blot, Mouse) Western Blot Mouse
Hiramitsu S, Terauchi M, Kubota T The effects of Dickkopf-4 on the proliferation, differentiation, and apoptosis of osteoblasts. Endocrinology, 2013-10-08;154(12):4618-26. 2013-10-08 [PMID: 24105477] (Western Blot, Mouse) Western Blot Mouse
Untergasser G, Steurer M, Zimmermann M, Hermann M, Kern J, Amberger A, Gastl G, Gunsilius E The Dickkopf-homolog 3 is expressed in tumor endothelial cells and supports capillary formation. Int. J. Cancer, 2008-04-01;122(7):1539-47. 2008-04-01 [PMID: 18033687] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol DKK3
Uniprot