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Cbx8 Antibody

Images

 
Western Blot: Cbx8 Antibody [NBP1-83224] - Analysis in human cell line A-549.
Immunocytochemistry/ Immunofluorescence: Cbx8 Antibody [NBP1-83224] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Analysis in human testis and liver tissues. Corresponding CBX8 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Cbx8 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LAVDTFPARVIKHRAAFLEAKGQGALDPNGTRVRHGSGPPSSVGGLYRDMGAQGGRPSLIARIPVARILGDPEEESWSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGF
Predicted Species
Mouse (95%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBX8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cbx8 Protein (NBP1-83224PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cbx8 Antibody

  • chromobox homolog 8 (Drosophila Pc class)
  • chromobox homolog 8 (Pc class homolog, Drosophila)
  • chromobox homolog 8
  • HPC3
  • Pc3
  • PC3chromobox protein homolog 8
  • Polycomb 3 homolog
  • polycomb 3
  • RC1Pc class 3 homolog
  • Rectachrome 1

Background

CBX8 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CBX8