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Dkk-1 Antibody (141135)

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Dkk-1 was detected in immersion fixed paraffin-embedded sections of human colon using Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Dkk-1 was detected in immersion fixed paraffin-embedded sections of human colon using Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ELISA(Cap)
Clone
141135
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB10962-SP
Carrier Free
MAB10962-500
100% Guarantee on every product

Dkk-1 Antibody (141135) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907
Specificity
Detects human Dkk-1 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Dkk-4, rhKremen-1, rhKremen-2 or rhLRP-6 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DKK1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
Application Notes
ELISA Capture: Human Dkk-1 Antibody (Catalog # MAB10962)
ELISA Detection: Human Dkk-1 Biotinylated Antibody (Catalog # BAF1096)
Standard: Recombinant Human Dkk-1 (Catalog # 5439-DK)
Publications
Read Publications using
MAB10962 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-1 Antibody (141135)

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Dkk-1 Antibody
(141135)
MAB10962
Dkk-1 Antibody (141135)
Species: Hu
Applications: IHC, ELISA(Cap)

Publications for Dkk-1 Antibody (MAB10962)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA Development, Neutralization.


Filter By Application
ELISA Development
(3)
Neutralization
(1)
All Applications
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Human
(3)
Mouse
(1)
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Showing Publications 1 - 5 of 5.
Publications using MAB10962 Applications Species
Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al. Role of Wnt/ beta -catenin and RANKL/OPG in bone healing of diabetic Charcot arthropathy patients Acta Orthopaedica 2015-07-21 [PMID: 25811776]
Tilman D Rachner, Andy Göbel, Stefanie Thiele, Martina Rauner, Peggy Benad-Mehner, Peyman Hadji et al. Dickkopf-1 is regulated by the mevalonate pathway in breast cancer Breast Cancer Research 2014-02-14 [PMID: 24528599]
Bajada S, Marshall MJ, Wright KT, Richardson JB, Johnson WE Decreased osteogenesis, increased cell senescence and elevated Dickkopf-1 secretion in human fracture non union stromal cells. Bone, 2009-06-18;45(4):726-35. 2009-06-18 [PMID: 19540374] (ELISA Development, Human) ELISA Development Human
Heider U, Kaiser M, Mieth M, Lamottke B, Rademacher J, Jakob C, Braendle E, Stover D, Sezer O Serum concentrations of DKK-1 decrease in patients with multiple myeloma responding to anti-myeloma treatment. Eur. J. Haematol., 2008-11-10;82(1):31-8. 2008-11-10 [PMID: 19067746] (ELISA Development, Human) ELISA Development Human
Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma. Blood, 2008-02-27;112(1):196-207. 2008-02-27 [PMID: 18305214] (ELISA Development, Neutralization, Human, Mouse) ELISA Development, Neutralization Human, Mouse

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Bioinformatics

Gene Symbol DKK1
Uniprot