Human DGAT1 - Ready-To-Use ELISA Kit (Colorimetric) Summary
Description |
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time. Assay Length: 3 hours |
Standard Curve Range |
0.312 - 20 ng/mL (example only; lot dependent) |
Sensitivity |
0.114 ng/mL (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 12 (example only; lot dependent) |
Intra-Assay |
%CV < 10 (example only; lot dependent) |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
DGAT1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Detection Solution A
- Detection Solution B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard Diluent
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Human DGAT1 - Ready-To-Use ELISA Kit (Colorimetric)
Background
Diglyceride acyltransferase (DGAT) is a membrane-bound Sterol o-acyltransferase subfamily member. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. Specifically, it catalyzes the terminal step in triacylglycerol synthesis, using diacylglycerol (DAG) and fatty acyl CoA as substrates.
DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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