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Desmin Antibody (1089115) [Unconjugated]

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Desmin was detected in immersion fixed paraffin-embedded sections of human Skeletal Muscle using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607) at 1ug/mL at 37 ° Celsius for 4 minutes. Before ...read more
Western Blot shows lysates of RD human rhabdomyosarcoma cell line, C2C12 mouse myoblast cell line and rat heart tissue. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # ...read more
Desmin was detected in immersion fixed paraffin-embedded sections of heart using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607) at 5 µg/ml for 1 hour at room temperature followed by incubation with ...read more
Desmin was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607) at 5 µg/ml for 1 hour at room temperature followed by ...read more
Desmin was detected in immersion fixed RD cells (Positive) and A172 human glioblastoma cell line (Positive) using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # mab11607) at 8 µg/ml for 3 hours at room ...read more
Simple Western shows lysates of RD human rhabdomyosarcoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Desmin at approximately 59 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Desmin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF, mIF
Clone
1089115
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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Desmin Antibody (1089115) [Unconjugated] Summary

Immunogen
E. coli derived recombinant human Desmin
Accession # P17661
Specificity
Detects recombinant human Desmin protein in Direct ELISA.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Multiplex Immunofluorescence 1 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Instructions
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmin Antibody (1089115) [Unconjugated]

  • CMD1I
  • CMD1IFLJ41013
  • CSM1
  • CSM2
  • DES
  • Desmin
  • FLJ12025
  • FLJ39719
  • FLJ41793
  • intermediate filament protein

Background

Desmin is a 53 kDa, muscle-specific molecule that belongs to the intermediate filament superfamily of cytoskeletal proteins. Cytoplasmic Desmin homopolymers form stable filamentous networks that stabilize lateral alignments of myofibrils. Human Desmin is 470 amino acid peptide. It contains an N-terminal head segment (aa 1‑84), multiple coiled regions (aa 109‑412) and a tail region (aa 413‑470). Single amino acid substitutions and three-amino acid deletions are associated with cardio‑skeletal myopathies. Most mutations affect filament assembly. Desmin is a highly conserved protein. Over the region used as immunogen, human Desmin is 99% identical to the corresponding mouse, canine and porcine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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