Novus Biologicals products are now on bio-techne.com

DDX25 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: DDX25 Antibody [NBP1-83328] - Staining in human testis and endometrium tissues using anti-DDX25 antibody. Corresponding DDX25 RNA-seq data are presented for ...read more
Western Blot: DDX25 Antibody [NBP1-83328] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-153
Immunohistochemistry-Paraffin: DDX25 Antibody [NBP1-83328] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: DDX25 Antibody [NBP1-83328] - Staining of human hypothalamus shows strong cytoplasmic positivity in subsets of glial cells and in processes.
Immunohistochemistry-Paraffin: DDX25 Antibody [NBP1-83328] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

DDX25 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NSHFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DDX25 Protein (NBP1-83328PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DDX25 Antibody

  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 25
  • DEAD box protein 25
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25
  • EC 3.6.1
  • EC 3.6.4.13
  • Gonadotropin-regulated testicular RNA helicase
  • GRTHATP-dependent RNA helicase DDX25

Background

DDX25, also known as ATP-dependent RNA helicase DDX25, consists of a 54.7 kDa and a 42 kDa isoform and is involved in regulation of translation and export of mRNA during development of spermatid. The protein has been utilized in current research of diseases and disorders such as pneumonia, malaria, male infertility, and azoospermia. The protein is found in RNA binding pathways in the testis, pituitary, and hypothalamus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

1670-SE
Species: Ec
Applications: EnzAct
NBP2-92630
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-07755
Species: Hu
Applications: WB
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
NBP2-34014
Species: Hu
Applications: IHC, IHC-P
NBP2-31637
Species: Hu
Applications: IHC, IHC-P
DSHBG0B
Species: Hu
Applications: ELISA
NBP1-54446
Species: Ch, Hu
Applications: ELISA, GS, ICC/IF, IP, WB
NB100-317
Species: Hu, Mu, Rt
Applications: ChIP, ChIP, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-71770
Species: Hu, Mu
Applications: ELISA, ICC/IF, IF, IHC, IHC-Fr, IHC-P, WB
NBP2-94256
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP2-01863
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AF2030
Species: Hu
Applications: IHC, WB
NBP1-82756
Species: Hu
Applications: IHC, IHC-P
MAB7428
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ICFlow, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NBP1-83328
Species: Hu
Applications: WB, IHC

Publications for DDX25 Antibody (NBP1-83328) (0)

There are no publications for DDX25 Antibody (NBP1-83328).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DDX25 Antibody (NBP1-83328) (0)

There are no reviews for DDX25 Antibody (NBP1-83328). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DDX25 Antibody (NBP1-83328) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DDX25 Products

Array NBP1-83328

Research Areas for DDX25 Antibody (NBP1-83328)

Find related products by research area.

Blogs on DDX25

There are no specific blogs for DDX25, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DDX25 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DDX25