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Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ha, Ma, Sh, Ch(-), Xp(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
SPM268
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

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Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 635] Summary

Immunogen
Cytoskeletal preparation from HeLa cells
Localization
Cytoplasmic
Specificity
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Chicken or Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 635]

  • cell proliferation-inducing protein 46
  • CK18
  • CK5
  • CYK18
  • DDD
  • K18
  • K1CO
  • K5
  • Keratin 18
  • Keratin 5
  • Keratin
  • KRT18
  • krt5
  • KRT5A
  • KRTB
  • type I cytoskeletal 18
  • type II cytoskeletal 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT5