![Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human urinary bladder epithelium](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Immunohistochemistry-Frozen-NBP1-97730-img0008.jpg "Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human urinary bladder epithelium")
| Reactivity | Hu, Mu, Rt, Po, Ca, Fe, Ha, RbSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | RCK102 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Immunogen | Derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with Cytokeratins from a Human lung cancer cell line (MR21). |
| Specificity | This antibody is a Cytokeratin antibody reacting with Cytokeratin 5 and Cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT5 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications. This antibody is CyTOF ready. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.2 |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 5/8 Antibody (NBP1-97730)Find related products by research area.
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| Gene Symbol | KRT5 |
![Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - An intracellular stain was performed on HeLa cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Flow-Cytometry-NBP1-97730-img0010.jpg "Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - An intracellular stain was performed on HeLa cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Immunohistochemistry-Frozen-NBP1-97730-img0006.jpg "Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes")
![Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes and bile ducts](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Immunohistochemistry-Frozen-NBP1-97730-img0007.jpg "Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes and bile ducts")
![Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on NTERA-2 cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-RCK102-Flow-Cytometry-NBP1-97730-img0009.jpg "Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on NTERA-2 cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")