![Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (C-50) [NBP2-44704] - Formalin-fixed. paraffin-embedded human colon carcinoma stained with cytokeratin 5/8 Monoclonal Antibody (C-50)](http://images.novusbio.com/fullsize/Cytokeratin-5-8-Antibody-C-50-Immunohistochemistry-Paraffin-NBP2-44704-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (C-50) [NBP2-44704] - Formalin-fixed. paraffin-embedded human colon carcinoma stained with cytokeratin 5/8 Monoclonal Antibody (C-50)")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ha, Ma, Sh, Ch(-), Xp(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, ICC/IF |
| Clone | C-50 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 0.5 ml at 200 ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47824) Store at 2 to 8C. |
| Immunogen | Cytoskeletal preparation from HeLa cells |
| Localization | Cytoplasmic |
| Specificity | It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT5 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS, pH 7.4 with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 5/8 Antibody (NBP2-44704)Find related products by research area.
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