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Cystatin A Antibody

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Genetic Strategies: Western Blot: Cystatin A Antibody [NBP1-86989] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is ...read more
Immunocytochemistry/ Immunofluorescence: Cystatin A Antibody [NBP1-86989] - Staining of human cell line hTCEpi shows localization to nucleus & cytosol.
Immunohistochemistry-Paraffin: Cystatin A Antibody [NBP1-86989] - Staining of human skin shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Western Blot: Cystatin A Antibody [NBP1-86989] - Analysis in human esophagus tissue.
Immunocytochemistry/ Immunofluorescence: Cystatin A Antibody [NBP1-86989] - Staining of human cell line U-2 OS shows positivity in nucleus and cytoplasm.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Cystatin A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSTA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cystatin A Protein (NBP1-86989PEP)
Publications
Read Publications using NBP1-86989.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cystatin A Antibody

  • AREI
  • CSTA
  • cystatin A (stefin A)
  • Cystatin A
  • cystatin AS
  • cystatin-A
  • Stefin A
  • STF1
  • STF1Cystatin-AS
  • STFA
  • STFAStefin-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cystatin A Antibody (NBP1-86989)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cystatin A Antibody (NBP1-86989) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CSTA