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Cystatin E/M/CST6 Antibody (216820)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP, B/N
Clone
216820
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB1284-SP
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Cystatin E/M/CST6 Antibody (216820) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin E/M
Glu29-Val149
Accession # NP_082899
Specificity
Detects mouse Cystatin E/M in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cystatin B, S, E/M, SA, S/N, recombinant mouse (rm) Cystatin A, C, D, rhFetuin A, B, rmHPRG, or rhKininogen is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CST6
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Neutralization 7 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin E/M/CST6 Antibody (216820)

  • CST6
  • cystatin 6
  • Cystatin E/M
  • cystatin M
  • cystatin M/E
  • Cystatin-6
  • cystatin-E
  • cystatin-M
  • cysteine proteinase inhibitor

Background

Cystatin E/M is a member of family 2 of the cystatin superfamily (1 ‑ 6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice with a null mutation in the cystatin E/M gene have defects in epidermal cornification and die between 5 and 12 days of age (7). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (8). In addition to being a cysteine protease inhibitor, the human cystatin E/M is also a substrate for transglutaminases (9).

  1. Carninci, P. and Y. Hayashizaki (1999) Meth. Enzymol. 303:19.
  2. Carninci, P. et al. (2000) Genome Res. 10:1617.
  3. Shibata, K. et al. (2000) Genome Res. 10:1757.
  4. Kawai, J. et al. (2001) Nature 409:685.
  5. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
  6. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
  7. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
  8. Hong, J. et al. (2002) J. Neurochem. 81:922.
  9. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cystatin E/M/CST6
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MAB1284
Cystatin E/M/CST6 Antibody (216820)
Species: Mu
Applications: WB, IP, B/N

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Bioinformatics

Gene Symbol CST6
Uniprot