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Cystatin D/Cst5 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF1202
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1202
Carrier Free
AF1202
Carrier Free
AF1202-SP
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Cystatin D/Cst5 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin D (R&D Systems, Catalog # 1202-PI)
Thr29-Val142
Accession # CAA49838
Specificity
Detects human Cystatin D in Western blots. In this format, approximately 10% cross-reactivity with recombinant human (rh) Cystatin C and rhCystatin SN is observed, less than 5% cross-reactivity with rhCystatin S is observed and less than 1% cross-reactivity with rhCystatin A, rhCystatin B and rhCystatin E/M is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CST5
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin D/Cst5 Antibody [Biotin]

  • CST5
  • CSTD
  • cystatin 5
  • Cystatin D
  • Cystatin-5
  • cystatin-D
  • cysteine-proteinase inhibitor
  • MGC71922

Background

Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).

  1. Freije, J.P. et al. (1993) J. Biol. Chem. 268:15737.
  2. Balbin, M. et al. (1994) J. Biol. Chem. 269:23156.
  3. Collins, A.R. and A. Grubb (1998) Oral Microbiol. Immunol. 13:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CST5
Uniprot