![Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-91020242014662.jpg "Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP46A1. The exact sequence is proprietary. |
| Localization | Endoplasmic reticulum membrane; Microsome membrane |
| Predicted Species | Rat (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CYP46A1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-16066 | Applications | Species |
|---|---|---|
| Biswas L, Zeng Z, Graham A, Shu X Gypenosides mediate cholesterol efflux and suppress oxidized LDL induced inflammation in retinal pigment epithelium cells Exp. Eye Res. 2020-01-10 [PMID: 31931003] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for CYP46A1 Antibody (NBP2-16066)Find related products by research area.
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![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202420104468.jpg "Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202419593547.jpg "Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")