![Immunocytochemistry/Immunofluorescence: Seladin 1 Antibody [NBP1-32247] - Analysis of HeLa, using Seladin 1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Seladin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32247-img0002.jpg "Immunocytochemistry/Immunofluorescence: Seladin 1 Antibody [NBP1-32247] - Analysis of HeLa, using Seladin 1 antibody at 1:200 dilution.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Seladin 1. The exact sequence is proprietary. |
| Localization | Endoplasmic reticulum membrane, Golgi apparatus membrane |
| Predicted Species | Porcine (95%), Bovine (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DHCR24 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
| Publication using NBP1-32247 | Applications | Species |
|---|---|---|
| Kohlhaas J, Jager M. A, et al. Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression. Exp Cell Res 2021-01-07 [PMID: 33422461] (Simple Western, Human) | Simple Western | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DHCR24 |
![Immunohistochemistry-Paraffin: Seladin 1 Antibody [NBP1-32247] - Paraffin-embedded rat adrenal gland. Seladin 1 antibody [N1C2] diluted at 1:500.](http://images.novusbio.com/fullsize/Seladin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-32247-img0006.jpg "Immunohistochemistry-Paraffin: Seladin 1 Antibody [NBP1-32247] - Paraffin-embedded rat adrenal gland. Seladin 1 antibody [N1C2] diluted at 1:500.")
![Immunohistochemistry-Paraffin: Seladin 1 Antibody [NBP1-32247] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Seladin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-32247-img0003.jpg "Immunohistochemistry-Paraffin: Seladin 1 Antibody [NBP1-32247] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.")
![Western Blot: Seladin 1 Antibody [NBP1-32247] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [N1C2] (NBP1-32247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32247_rabbit-polyclonal-seladin-1-antibody-9102024205630.jpg "Western Blot: Seladin 1 Antibody [NBP1-32247] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [N1C2] (NBP1-32247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Seladin 1 Antibody [NBP1-32247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [N1C2] (NBP1-32247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32247_rabbit-polyclonal-seladin-1-antibody-910202420134081.jpg "Western Blot: Seladin 1 Antibody [NBP1-32247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [N1C2] (NBP1-32247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Seladin 1 Antibody [NBP1-32247] - Various whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [N1C2] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32247_rabbit-polyclonal-seladin-1-antibody-1610202419405729.jpg "Western Blot: Seladin 1 Antibody [NBP1-32247] - Various whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [N1C2] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
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![Western Blot BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images2/BRCA2_MAB2476_Western_Blot_6033.jpg)
![Immunohistochemistry BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images2/mab2476_human-brca2-mab-clone-234403-immunohistochemistry-10520211657.jpg)
![Western Blot BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images/mab2476_human-brca2-mab-clone-234403-2282024172111.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")