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CYP21A2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Analysis in human adrenal gland and kidney tissues using NBP2-38698 antibody. Corresponding CYP21A2 RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human adrenal gland, kidney, pancreas and testis using Anti-CYP21A2 antibody NBP2-38698 (A) shows similar protein ...read more
Western Blot: CYP21A2 Antibody [NBP2-38698] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue
Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells in adrenal cortex.
Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human kidney shows no positivity in cells in tubules as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CYP21A2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP21A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Simple Western reported by internal validation
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western internal validation: Separated by size; matrix was 12-230 kDa; detected by Chemiluminescence.
Control Peptide
CYP21A2 Protein (NBP2-38698PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYP21A2 Antibody

  • 21-OHase
  • CA21HCYP21B
  • CAH1
  • CPS1
  • CYP21
  • CYP21A2
  • CYP21B
  • CYP21EC 1.14.99.10
  • Cytochrome P450 21
  • Cytochrome P450 XXI
  • cytochrome P450, family 21, subfamily A, polypeptide 2
  • cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenalhyperplasia), polypeptide 2
  • Cytochrome P-450c21
  • Cytochrome P450-C21
  • Cytochrome P450-C21B
  • EC 1.14.99
  • MGC150536
  • MGC150537
  • P450c21B
  • steroid 21-hydroxylase
  • steroid 21-monooxygenase

Background

The CYP21A2 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins aremonooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids andother lipids. This protein localiz

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYP21A2
Uniprot