![Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NB600-495-img0005.jpg "Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.")
| Reactivity | Hu, BvSpecies Glossary |
| Applications | WB, ELISA, IHC, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody was prepared from monospecific antiserum by delipidation and defibrination Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Cyclin A2 Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A. (Uniprot: P20248) |
| Predicted Species | Bovine (90%). Backed by our 100% Guarantee. |
| Isotype | Serum |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCNA2 |
| Purity | Delipidation and Defibrination |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by western blot and immunohistochemistry and is suitable for the detection by immunoblot, ELISA, IHC, and IP of human, rat and mouse Cyclin A. Researchers should determine optimal titers for applications that are not stated below. |
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| Control |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Antiserum |
| Preservative | 0.01% Sodium Azide |
| Purity | Delipidation and Defibrination |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin A2 Antibody (NB600-495)Find related products by research area.
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![Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495] - Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NB600-495-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495] - Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.")
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