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Cyclin A1 Antibody (722407) [Unconjugated]

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Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin A1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, MCF-7 human breast cancer cell ...read more
Cyclin A1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
722407
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB7046-SP
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Cyclin A1 Antibody (722407) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Cyclin A1
Lys51-Arg220
Accession # P78396
Specificity
Detects human Cyclin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cyclin A2, B1, B2, C, D1, D2, D3, E1, E2, or recombinant mouse Cyclin A1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCNA1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB7046 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclin A1 Antibody (722407) [Unconjugated]

  • CCNA1
  • Cyclin A1
  • cyclin-A1

Background

Cyclin A1, also known as CCNA1, is a 64 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression, apoptosis, double strand DNA break repair, and mRNA splicing. Cyclin A1 activity promotes G2/M phase transition in spermatocyte meiosis and G1/S transition in somatic cells. Cyclin A1 is upregulated in myeloid leukemia, testicular germ cell tumors, and several carcinomas. It contains two cyclin box folds (aa 243-318 and 340-427). Over aa 51-220, human Cyclin A1 shares approximately 75% aa identity with mouse and rat Cyclin A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cyclin A1 Antibody (722407) [Unconjugated]
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Publications for Cyclin A1 Antibody (MAB7046)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol CCNA1
Uniprot