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CTGF/CCN2 Antibody

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Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426] - Paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary ...read more
Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426] - (6ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Control: CTGF/CCN2 Antibody [NBP2-66426] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications ELISA, ICC/IF, IHC, Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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CTGF/CCN2 Antibody Summary

Immunogen
Peptide with sequence C-SLYYRKMYGDMA, from the C Terminus of the protein sequence according to NP_001892.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCN2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Control
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Immunohistochemistry 6.0 - 8.0 ug/ml
  • Peptide ELISA Detection limit 1:10000
Application Notes
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells.

Reactivity Notes

Expected from sequence similarity: Human, Rat and Canine

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Antigen Affinity-purified

Alternate Names for CTGF/CCN2 Antibody

  • CCN2
  • CCN2IGFBP-8
  • connective tissue growth factor
  • CTGF
  • CTGRP
  • Fisp12
  • HCS24
  • Hypertrophic chondrocyte-specific protein 24
  • IBP-8
  • IGF-binding protein 8
  • IGFBP-8
  • IGFBP8CCN family member 2
  • Insulin-like growth factor-binding protein 8
  • MGC102839
  • NOV2

Background

Connective Tissue Growth Factor (CTGF) is a 38kDa, cysteine-rich, secreted peptide. CTGF promotes endothelial cell growth, migration, adhesion and survival. and is thus implicated in endothelial cell function and angiogenesis. CTGF is implicated in are embryogenesis, wound healing and regulation of extracellular matrix production, and is required for normal skeletal growth.

CTGF antibodies are useful tools for angiogenesis and cell structure research, and for studies on certian types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for CTGF/CCN2 Antibody (NBP2-66426). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
    • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
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Bioinformatics

Gene Symbol CCN2