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CS Citrate Synthase Antibody (CL2553)

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Western Blot: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in MCF7 cell line with Anti-CS monoclonal antibody, showing specific mitochondrial staining in green. Microtubule- ...read more
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in HeLa cell line showing specific mitochondrial staining in green. Microtubule-staining and nuclear probes are ...read more
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human cell line A431 showing specific mitochondrial staining in green. Microtubule-staining and nuclear probes ...read more
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in U2OS cell line with Anti-CS monoclonal antibody, showing specific mitochondrial staining in green. ...read more
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in U251 cell line showing specific mitochondrial staining in green. Microtubule-staining and nuclear probes are ...read more
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human fallopian tube shows strong granular cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human breast cancer shows strong granular cytoplasmic positivity in tumor cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human rectum shows strong granular cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human small intestine shows granular cytoplasmic immunoreactivity in both glandular and lamina propria cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human prostate shows granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL2553
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CS Citrate Synthase Antibody (CL2553) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:ADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWL
Epitope
GLFWLLVTGHIPTEE
Predicted Species
Mouse (93%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CS
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for CS Citrate Synthase Antibody (CL2553)

  • citrate (Si)-synthase
  • citrate synthase
  • citrate synthase, mitochondrial
  • CS Citrate Synthase
  • EC 2.3.3
  • EC 2.3.3.1

Background

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CS
Uniprot