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CREB [p Ser133] Antibody

Images

 
Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). ...read more
Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu, Bv, Ca, Ch, Pm, Sh, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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CREB [p Ser133] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH. Accession # P15337
Modification
p Ser133
Localization
Nuclear
Specificity
Specific for endogenous levels of the ~45 kDa CREB protein phosphorylated at Ser133. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Predicted Species
Human (100%), Sheep (100%), Canine (100%), Chicken (100%), Primate (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CREB1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000
  • Western Blot 1:1000
Application Notes
This antibody has not been validated for use in paraffin embedded sections (IHC-P). For IHC-Fr, the antibody was used at 1:1000 and incubated for 48 hours at 4C. The dilution buffer used was 0.1M PBS, 0.5% Triton-100, and 3% normal serum. No antigen retrieval step was performed.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-273 in the following applications:

  • WB
    4 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34346292). Mouse reactivity reported in scientific literature (PMID: 21838783). .

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for CREB [p Ser133] Antibody

  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • cAMP-responsive element-binding protein 1
  • CREB
  • CREB1
  • CREB-1
  • cyclic AMP-responsive element-binding protein 1
  • MGC9284
  • pCREB
  • transactivator protein

Background

It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogenactivated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs)phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CREB Antibody (NB300-273)(10)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
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Mouse
(1)
Rat
(3)
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Showing Publications 1 - 10 of 10.
Publications using NB300-273 Applications Species
Costa DVS, Shin JH, Goldbeck SM et al. Adenosine receptors differentially mediate enteric glial cell death induced by Clostridioides difficile Toxins A and B Frontiers in immunology 2023-01-16 [PMID: 36726986] (WB, Rat)

Details:
Dilutions: 1:500
WB Rat
Strand E, HollAs H, Sakya SA Et al. Annexin A2 binds the internal ribosomal entry site of c-myc mRNA and regulates its translation RNA biology 2021-08-04 [PMID: 34346292] (WB, Rat) WB Rat
Wall AM, Corcoran AE, O'Halloran KD, O'Connor JJ. Effects of prolyl-hydroxylase inhibition and chronic intermittent hypoxia on synaptic transmission and plasticity in the rat CA1 and dentate gyrus. Neurobiol Dis. 2013-09-17 [PMID: 24055213] (WB, Rat) WB Rat
Schneider A, Mehmood T, Pannetier S, Hanauer A. Altered ERK/MAPK signaling in the hippocampus of the mrsk2_KO mouse model of Coffin-Lowry syndrome. J Neurochem. 2011-08-12 [PMID: 21838783] (WB, Mouse) WB Mouse
Johannessen M, Delghandi MP, Moens U. What turns CREB on? . Cell Signal;16(11):1211-27. 2004-11-01 [PMID: 15337521]
Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia ZG, Greenberg ME. CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34:221-233. 2002-01-01 [PMID: 11970864]
Shaywitz AJ, Greenberg ME. CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 68:821-861. [PMID: 10872467]
Silva AJ, Kogan JH, Frankl PW, Kida S. CREB memory. Annu Rev Neurosci 21:127-148. 1998-01-01 [PMID: 9530494]
Montminy M. Transcriptional regulation by cyclic AMP. Annu Rev Biochem 66:807-822. 1997-01-01 [PMID: 9242925]
Karin M, Hunter T. Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus. Curr Biol 5:747-757. [PMID: 7583121]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CREB Antibody (NB300-273). (Showing 1 - 1 of 1 FAQs).

  1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
    • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Secondary Antibodies

 

Isotype Controls

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Research Areas for CREB Antibody (NB300-273)

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Blogs on CREB.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Bioinformatics

Gene Symbol CREB1
Entrez
Uniprot