CREB Antibodies
We offer CREB Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), ELISA, Gel Super Shift Assays, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each CREB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CREB Antibodies can be used in a variety of model species: Human, Monkey, Mouse, Primate, Rat. Use the list below to choose the CREB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CREB polyclonal antibodies and browse our CREB monoclonal antibody catalog.
Alternate Names for CREB Antibodies
anti-CREB antibody, anti-CREB1 antibody, anti-active transcription factor CREB antibody, anti-cAMP responsive element binding protein 1 antibody, anti-cAMP-response element-binding protein-1 antibody, anti-cAMP-responsive element-binding protein 1 antibody, anti-CREB-1 antibody, anti-cyclic AMP-responsive element-binding protein 1 antibody, anti-MGC9284 antibody, anti-transactivator protein antibody
18 Results for "creb" in Primary Antibodies
![Western Blot: CREB Antibody [NBP1-90364] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NBP1-90364-img0006.jpg "Western Blot: CREB Antibody [NBP1-90364] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP1-90364] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90364-img0007.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP1-90364-img0002.jpg)
![Western Blot: CREB Antibody [NBP1-90364] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NBP1-90364-img0005.jpg)
![Simple Western: CREB Antibody [NBP1-90364] - Human foreskin cell lysate. CREB antibody dilution of 1:50. Protein concentration is 750 ug/mL. Detection is chemiluminescence. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CREB-Antibody-Simple-Western-NBP1-90364-img0008.jpg)
![Simple Western: CREB Antibody [NBP1-90364] - Detection of CREB by Simple Western (JESS) in reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody at 1:50. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CREB-Antibody-Simple-Western-NBP1-90364-img0009.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Notch1 affects CREB phosphorylation and transcription. Immunoblot on whole hippocampal lysates shows a reduction in CREB phosphorylation in N1cKO as compared to WT (n = 3 independent experiments). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/Article/10.3389/fncel.2015.00447/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0026.jpg "Western Blot: CREB Antibody (LB9) [NB100-74393] - Notch1 affects CREB phosphorylation and transcription. Immunoblot on whole hippocampal lysates shows a reduction in CREB phosphorylation in N1cKO as compared to WT (n = 3 independent experiments). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/Article/10.3389/fncel.2015.00447/abstract) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0019.jpg)
![Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Staining of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:400 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0025.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0021.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Analysis of HepG2 cells](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0022.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Cancer biopsies of deparaffinized human bladder and rectal tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0018.jpg)
![Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye. Images were taken on a Thermo Scientific ArrayScan at 20X magnification.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0024.jpg)
![Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB was performed on untreated 293T cells. Antigen: antbody complex was formed by binding 500ug whole cell lysate with 2ug of mouse monoclonal antibody recognizing CREB overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:1000 overnight rotating at 4C. Membranes were then washed in TBST and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunoprecipitation-NB100-74393-img0023.jpg)
![Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).](http://images.novusbio.com/fullsize/CREB-[p-Ser133]-Antibody-Western-Blot-NB300-273-img0007.jpg "Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).")
![Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.](http://images.novusbio.com/fullsize/CREB-[p-Ser133]-Antibody-Immunohistochemistry-Frozen-NB300-273-img0006.jpg)
![Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NBP2-22516-img0001.jpg "Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.")
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0004.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human glioma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0007.jpg)
![Chromatin Immunoprecipitation: CREB Antibody [NBP2-22516] - Analysis performed using cross-linked chromatin from colon carcinoma cells treated with serum. IP was performed using a multiplex microplate Matrix ChIP assay of a CREB polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data one in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2 of Egr1.Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the Egr-1 locus is shown; xes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/CREB-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-22516-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of mouse brain tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0005.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human lung adenocarcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0006.jpg)
![Western Blot: CREB Antibody (5G3) [NBP2-37610] - Analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Western-Blot-NBP2-37610-img0002.jpg "Western Blot: CREB Antibody (5G3) [NBP2-37610] - Analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CREB Antibody (5G3) [NBP2-37610] - Analysis of Hela cells using CREB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Immunocytochemistry-Immunofluorescence-NBP2-37610-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody (5G3) [NBP2-37610] - Analysis of prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Immunohistochemistry-NBP2-37610-img0004.jpg)
![ELISA: CREB Antibody (5G3) [NBP2-37610] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-ELISA-NBP2-37610-img0001.jpg)
![Immunohistochemistry: CREB [p Ser 133] Antibody (SR1800) [NBP3-21507] - Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using NBP3-21507.](http://images.novusbio.com/fullsize/nbp3-21507_rabbit-creb-p-ser-133-mab-sr1800-57202316494290.jpg "Immunohistochemistry: CREB [p Ser 133] Antibody (SR1800) [NBP3-21507] - Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using NBP3-21507.")
![Western Blot: CREB [p Ser 133] Antibody (SR1800) [NBP3-21507] - Western blot analysis using NBP3-21507 in HeLa cell lysates treated with Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21507_rabbit-creb-p-ser-133-mab-sr1800-6720239533770.jpg)
![Western Blot: CREB Antibody [NB100-87001] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-87001 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NB100-87001-img0004.jpg "Western Blot: CREB Antibody [NB100-87001] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-87001 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")
![Immunohistochemistry-Paraffin: CREB Antibody [NB100-87001] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CREB used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-NB100-87001-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23244_mouse-creb-p-ser133-mab-m415-19920231915415.jpg "Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23244_mouse-creb-p-ser133-mab-m415-199202312523628.jpg)