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Connexin 43/GJA1 Antibody

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Western Blot: Connexin 43/GJA1 Antibody [NB100-81867] - Extracts from HT29 and HepG2 cells using Connexin 43 (Ab-367) antibody
Western Blot: Connexin 43/GJA1 Antibody [NB100-81867] - Extracts from HT29 and HepG2 cells using Connexin 43 (Ab-367) antibody
Immunohistochemistry-Paraffin: Connexin 43/GJA1 Antibody [NB100-81867] - Paraffin-embedded human breast carcinoma tissue, using NB100-81867 (left) or the same antibody preincubated with blocking peptide (right).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Connexin 43/GJA1 Antibody Summary

Immunogen
Peptide sequence around amino acids 366~370 (R-A-S-S-R) derived from Human Connexin 43/GJA1.
Marker
Gap Junctions Marker
Specificity
Detects endogenous level of total Connexin 43/GJA1 protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GJA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Connexin 43/GJA1 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Connexin 43/GJA1 Antibody

  • Connexin 43
  • connexin-43
  • CX43
  • CX43gap junction protein, alpha-like
  • DFNB38
  • Gap junction 43 kDa heart protein
  • gap junction alpha-1 protein
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha 1, 43kDa
  • GJA1
  • GJAL
  • HSS
  • ODD
  • ODDD
  • ODOD
  • SDTY3

Background

Connexin 43 aka GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Connexin 43/GJA1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Blogs on Connexin 43/GJA1.

Check out the latest blog posts on Connexin 43/GJA1.
Connexin: Bridging the Gap of Intercellular Communication
Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a...  Read full blog post.
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Bioinformatics

Gene Symbol GJA1
Entrez
Uniprot