![Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment.](http://images.novusbio.com/fullsize/Connexin-43-GJA1-[p-Ser368]-Antibody-Western-Blot-NB100-722-img0001.jpg "Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment.")
| Reactivity | Mu, Rt, Hu, Bv, Ca, Ch, Gp, ZeSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH. Accession # P08050 |
| Modification | p Ser368 |
| Localization | Cytoplasmic and Integral membrane protein |
| Marker | Gap Junctions Marker |
| Specificity | Specific for endogenous levels of the ~43 kDa Connexin 43/GJA1 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with lambda-phosphatase. |
| Predicted Species | Human (100%), Chicken (100%), Canine (100%), Bovine (100%), Guinea Pig (100%), Zebrafish (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GJA1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~43 kDa. |
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| Theoretical MW | 43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Connexin 43/GJA1 Antibody (NB100-722)Find related products by research area.
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Connexin: Bridging the Gap of Intercellular Communication Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a... Read full blog post. |
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